Home / Reconstitution Calculator
Free research tool · For research use only

Peptide Reconstitution Calculator

Enter your research vial size, the bacteriostatic water you're adding, and the amount you want to measure — get the exact concentration and syringe units instantly. A laboratory measurement tool, not dosing guidance.

Research use only

Your reconstitution

All fields update the results live. Presets are common research vial sizes — enter any custom value in the box.

Your result
Draw to this mark on the syringe
50 units
= 0.50 mL on a U-100 insulin syringe
2.5 mg/mL
Concentration
25 mcg/u
Per syringe unit
20
Draws per vial
0.50 mL
Volume per draw
Fill line on your 0.3 mL syringe
030u
What this tool is. Reconstitution is the lab step of dissolving a lyophilized (freeze-dried) research peptide in bacteriostatic water so it can be measured accurately. This calculator does that measurement math only — concentration and the volume/units that correspond to a target amount. It is not a dosing protocol, not medical advice, and not a suggestion to administer anything. All referenced compounds are for laboratory and research use only — not for human consumption.

How the math works

Every result comes from three simple steps — no magic:

Step 1 — Concentration

Divide the peptide in the vial by the water you added.
concentration (mg/mL) = vial mg ÷ water mL
Example: 5 mg ÷ 2 mL = 2.5 mg/mL (2,500 mcg/mL).

Step 2 — Volume for your target amount

Divide the amount you want by the concentration.
volume (mL) = target mcg ÷ concentration (mcg/mL)
Example: 250 mcg ÷ 2,500 mcg/mL = 0.10 mL.

Step 3 — Syringe units

On a U-100 insulin syringe, 1 unit = 0.01 mL, so multiply volume by 100.
units = volume (mL) × 100
Example: 0.10 mL × 100 = 10 units.

Common questions
What is bacteriostatic water?

Bacteriostatic water is sterile water with 0.9% benzyl alcohol added to inhibit bacterial growth, which is why it's the standard diluent for reconstituting research peptides in a lab setting. The amount you add sets your concentration — more water means a lower concentration per unit.

Why insulin units instead of mL?

Research peptide volumes are tiny — often a fraction of a milliliter — so U-100 insulin syringes (marked in units, where 100 units = 1 mL) give far more precise, readable measurements than a standard mL syringe.

Is this a dosing recommendation?

No. This is a measurement/dilution calculator only. It converts numbers you enter into a concentration and a corresponding volume. It does not recommend an amount, and nothing on this site is medical advice. All compounds referenced are strictly for laboratory and research use — not for human consumption.

Where do I buy tested research peptides?

See our independent, COA-focused vendor rankings — we score every research-peptide company on purity, third-party Certificates of Analysis, reputation, and shipping.

See the vetted vendor rankings →